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The Barriers of the Central Nervous System (CNS) are a system of regulatory interfaces between the blood and the brain that are essential to brain function and have major impact on the course and treatment of many CNS diseases. The 2012 Barriers of the CNS Gordon Research Conference will present the latest, cutting edge research on the cellular and molecular mechanisms that comprise the CNS barriers and will explore state-of-the-art strategies for imaging barrier function and improving drug delivery for the treatment of human disease.