ИСТИНА

The brief description the International Workshop “High-Temperature Biotechnology” On 16 - 23 August 1995, an international workshop “High-Temperature Biotechnology” was conducted in Petropavlovsk-Kamchatsky (Russia). The organizers of the Workshop were Institute of Volcanology (under Russian Academy of Sciences), the Lomonosov Moscow State University (under the Ministry of Scientific and Technological Policy of Russian Federation), Moscow University Foundation “Alma-Mater”. The sponsors of the Workshop were Amersham Life Science Inc., Boehringer Mannheim GmbH, Elba Foundation, Finnzymes Oy,Genencor Int., Novo Nordisk, Roche Molecular Systems, Inc. The Workshop was attended by 35 researchers from various countries: Russia, USA, Germany, Austria, France, Belgium Great Britain, Denmark, Finland, New Zealand and Italy. The main topics to be covered are the following: • High-temperature microorganisms and microbial associations; • Thermostable enzymes of thermophiles; • Genetic engineering of thermophilic enzymes; • Transfer of genes into mesophiles; • Genetic engineering of thermophiles; New methods for genetic modification of thermophiles; • Biotechnological processes based on thermophiles and their enzymes; There were 19 scientific presentations. The Workshop Programme included the helicopter excursions to Uzon volcano caldera and Geysers Valley and a bus excursion to hydrothermal fields of Mutnovka volcano. The Programme is attached. PROGRAMME August 17, Thursday Cochairs: G.A. Karpov S.D. Varfolomeyev 9.00 Opening 10.00 E.A. Bonch-Osmolovskaya New Thermophilic Microorgannisms from Kamchatka Hydrothermal Areas. Coffee-break 11.30 H.W.Morgan Description of a New Isolate of Alkaliphilic Aerobic Thermophile. Lunch Cochairs: E.A. Bonch-Osmolovskaya H.W.Morgan 15.00 M. Klingeberg Isolation, Taxonomy and Genetics on Extreme Thermophilic Starch Degrading Microorganisms. 16.00 T.G. Sokolova Diversity and Distribution of Thermophilic Carboxydotrophic Anaerobic Bacteria. 17.00 R. Sharp Bioreactor and Media Optimisation for the Growth of Hyperthermophiles. August 18, Friday Field excursion August 19, Saturday Cochairs: F.T. Robb G.S. Monastyrskaya 9.00 S.D. Varfolomeyev High-Themperature Biocatalysis 10.00 W.H. Schwarz Hydrolysis of Hemicellulose by Thermostable Recombinant Enzymes Derived from the Thermophilic, Anaerobic Bacteria Clostridium thermocellum and Clostridium stercorarium. Coffee-break 11.30 T.P. Mosolova Thermostable Cellulases from the Clostridium Thermocellum Cellulase System. Lunch Cochairs: N.F. Myasoedov W.H. Schwarz 15.00 C. Nicolini Heat-Proof Enzymes in Langmuir-Blodgett Monolayers. Mesophilic Versus Thermophilic Thioredoxin: Functional and Structural Studies in Solution. 16.00 E.I. Rainina Immobilized thermophilic cells. 17.00 R. Cunin Survey of Aspartate Transcarbamylases from Thermophiles August 20, Sunday Field excursion August 21, Monday Cochairs: E.D. Sverdlov C. Nicolini 9.00 D.H. Gelfand Biochemical Properties of Thermophilic DNA Polymerases: Applications for PCR. 10.00 S.V. Kostrov Cloning of Genes from Thermophilic Microbial Strains and Analysis of the Molecular Base of Protein's Stability at the Model of Proteolytic Enzymes. Coffee-break 11.30 C. Schleper Characterization of Genetic Elements from Sulfolobus and their Use in Demonstrating Transformation of an Extremely Thermophilic Archaeon. 12.30 N.G. Belogurova C. thermosaccharolyticum Plasmids as Potential Cloning Vectors for Clostridia. 13.30 P. Mattila Cloning, Overexpression and Characterization of a Thermophilic DNA Polymerase from Thermus Brockianus Strain Isolated from Icelandic Hot-Springs. Lunch Cochairs: S.V. Kostrov D.H. Gelfand 15.00 W. Ankenbauer ExpandsTM PCR System: Better Way of PCR. 16.00 F.T. Robb A Molecular Genetic Approach to Studing Protein Function at High Temperature and Pressure. 17.00 Closing