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Western Dvina is a transboundary river, with a complex multifactorial hydrological and hydrochemical regime. The landscape features of Russian part of the watershed increase natural nutrients and organic matter content in river water. The main sources of water pollution are insufficiently treated municipal sewage and diffusion surface runoff from urban and agricultural areas. The aim of the study is an assessment of seasonal variability of hydrochemical indicators in Western Dvina and its tributaries.