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In this study, a new implementation of optical imaging of luminescent carbon multi-functional medical nano-agents in biomaterial using wavelet neural networks is proposed. It is demonstrated that this approach allows simultaneous detection of graphene oxide based drug carrier nanocomposites and their components against the background of autofluorescence of human urine with a sufficiently low detection threshold by concentration. The results are compared to similar results obtained by the authors using perceptron type artificial neural networks. The solution of this inverse problem of luminescence spectroscopy can be important for detection and quantitative evaluation of the content of several different luminescent nanoparticles in blood, various organs, in the cells.