ИСТИНА

The systematic evolution of ligands by exponential enrichment (SELEX) process is a combinatorial chemistry method for the isolation of nucleic acid ligands (aptamers) that bind to a desired target molecule with high affinity. Recombinant hIL6 was immobilized by CM5 chip. For four rounds of DNA combinatorial library selection to hIL6 we were isolated a pool of aptamers using surface plasmon resonance method, that was converted to double-strand DNA, amplified by PCR and subcloned into a pUC 19 with the SmaI restriction site. Individual clones were sequenced by the dideoxy method using standard protocols and method. Sequences were aligned relative to the consensus sequences. Some of them had high affinity to IL6. One of the aptamers turned out to be able binding hIL6 with apparent dissociation constant 0.51 ± 0.02 µM.