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Humic substances (HSs) actively adsorb toxicants from solutions (Perminova et al., 2006). НSs can be adsorbed on the cell bacterium surface and minerals for this reason they are decrease impact and bioavailability of different substances in the solutions. НSs are adsorbed on the cell surfaces of microorganisms (Fein et al.,1999, Tikhonov et al., 2013) and minerals (Zhou et al., 2001), reducing the effects and bioavailability of various substances in solutions. It has been shown that under adsorption of HSs on goethite fractionation occurs. HSs of low molecular weight were adsorbed as the first components, which were quickly replaced by high molecular weight fractions (Zhou et al., 2001). HSs with the high molecular weight were sorbed onto Bacillus subtilis predominantly (Maurice et al., 2004). There is little information about adsorption onto bacterial cells, collected from the different habitat. The aim of the study was to research the fractionation of HSs on bacterial cells isolated from natural waters.