ИСТИНА

Nanomaterials have received much attention due to their novel chemical, physical and electrical properties. Within separation science, gold nanoparticles have found a promising home, because of their long-term stability, high surface area-to-volume ratio, and ease of chemical modification. A new sorbent for HPLC based on a styrene-divinylbenzene copolymer (CSD) was synthesized by adsorption of gold nanoparticles on its surface, followed by modification through the formation of self-assembled monolayers by the reaction between gold and sulfur-containing reagent – lipoic acid with grafted lysine. Low- temperature nitrogen adsorption, diffuse reflection spectroscopy, scanning electron microscopy were used to examine physical and chemical properties of obtained sorbent. The chromatographic behavior of profens and β-blockers was studied on the sorbents depending on the composition of the mobile phase: the nature of the buffer solution, its concentration and pH, the proportion and nature of organic solvent. It was established that significant retention factors for both groups of substances are observed using the mixture of acetonitrile and buffer solution as the eluent. Herewith profens are kept longer with a lower content of organic solvent, and β-blockers, on the contrary. Hydrophobic interactions play a significant role in the retention of the studied compounds. The order of elution of profens correlates with their hydrophobicity indices logP, logD. This dependence is traced to a lesser extent for β-blockers, due to the additional interactions. The separation of the six-component mixture of β- blockers in 15 minutes and of the three-component mixture of profens in 20 minutes was carried out on the new sorbent.