ИСТИНА

The aim of the work is the synthesis of Sn (IV) c carboxylates based on bile acids and the study of their cytotoxic activity. Organotin carboxylates of the general formula R3Sn(LCOO) and R2Sn(LCOO)2 were synthesized (where R = Me, Ph; L is fragments of cholic (L1), deoxycholic (L2), lithocholic acid (L3). Compounds were characterized by 1H and 13C NMR and elemental analyzes. Cytotoxicity activity in vitro of the compounds was studied on A549, WI38, MCF-7, SW480, and HCT-116 cancer cell lines in the MTT test. It was shown that all compounds posess high cytotoxicity, IC50 values in the range of 0.2 - 8 μM were found. The toxicity of R2Sn(L2COO)2 is one order lower than that of R3Sn(L2COO) (1.3 and 0.3 μM, respectively). The study of the cell cycle and by flow cytometry showed that the compounds block the cell cycle in the G2/M phase. Analysis of the apoptotic profile showed that diphenyltin derivatives induce apoptosis more intensively than triphenyl derivatives (the number of apoptotic cells was 25% and 21%, respectively) (Fig. 1).