ИСТИНА

Oxidative stress participates in a pathogenesis of many diseases. 2,6-Di-alkylphenols are widely used as antioxidants and stabilizers of cellular membranes. On the other hand, pyridines are engaged in structures of many biologically active compounds. New substituted picolylamines with 2,6-di-tert-butyl-4-hydroxyphenyl groups were synthesized and characterized by IR, NMR, X-ray and elemental analysis. Antioxidant potential of compounds was studied as an inhibition of lipid peroxidation (LP) in rat liver homogenates. To elucidate the possible mechanisms of antioxidant activity the radical scavenging activity (in DPPH-test), superoxide radical anion O2-∙ scavenging activity (xanthine-xanthine oxidase assay), reducing ability (CUPRAC-test) and inhibition of lipoxygenase (LOX 1-B) were evaluated. All the compounds showed high antioxidant activity depending on the length of the linker and the position of nitrogen atom in the pyridine ring. Moreover, compounds possess moderate superoxide radical anion scavenging activity and act as mild inhibitors of lipoxygenase. Thus, studied compounds can be considered as potential antioxidants and cytoprotectors.