ИСТИНА

Blood analysis is an important field in the biomedical physics. Single-particle technique, such as flow cytometry, can provide high sensitivity and statistically significant blood analysis. However, typical commercial flow cytometry devices have known limitations such as two measured optical signals (FSC and SSC); it is enough to determine only the size and the refractive index of analyzed cells without any detailed information about the morphology and the shape. But some medicine applications requires more sophisticated methods and scanning flow cytometry technique (SFC) can be good alternative. This method allows the measurement of entire regular and polarized light-scattering patterns (LSP) from 10° to 70° polar angles. Solution of the inverse light-scattering (ILS) problem for SFC data exhibits unique information about leukocytes, red blood cells, blood platelets and blood microparticles. We aimed to expand the range of LSP with back scattering hemisphere (110°-170°) designing new optics and fluidics. Experimental setup was able to measure LSP for forward and backward hemisphere. Original flow chamber design shows adequate workability. We measured standard and back hemisphere LSP for polystyrene microspheres (d=4μm). Size and refraction index determination shows that additional signal such as back hemisphere LSP significantly increases precision for solution of the ILS problem.