ИСТИНА

Ginseng saponins are used in Traditional Chinese Medicine for more than 2000 years. Developed analytical method uses mass spectrometric detection in selected ion monitoring mode for sapogenin fragmentation ions of less polar ginsenosides in urine after ginseng infusion administration. Main advantage of this technique is the ability to detect simultaneously protopanaxatriol and protopanaxatriol derivatives and its deshydroxy analogues. Limits of detection were on the level of 0.01-0.02 mg/L. Good linearity and reproducibility were observed for this method. Developed approach was applied for pharmacokinetics study of ginsenosides less polar metabolites in healthy volunteers after ginseng infusion administration.