ИСТИНА

Online discussion: https://sfmconference.org/sfm/sfm22/conferences_workshops/internet-biophotonics-xv/preliminary/1573/#discussion Online presentation: https://sfmconference.org/files/orig/s/f/sfm-2-perevedentseva-1.pdf The utilization of the optical – scattering and spectroscopic – properties of diamond nanoparticles (nanodiamonds, ND) for bio-imaging/monitoring and bio-sensing is developed and often combined with additional ND functionalities like drug delivery and other treatments currently. Newly found property of NDs important for their multifunctional theranostic abilities is antimicrobial activity [1]. The use of the antibacterial properties of nanostructures is currently considered as an alternative to the antibiotics. In the presented work the activity of ultradispersed nanodiamonds of various origin was tested against pathogenic microorganisms – gram-negative and the gram-positive bacteria and for the first time against the fungi. The antimicrobial activity of ultradispersed NDs at the concentration of 500 µg/mL was assessed by the agar diffusion method [2] with the test strains of gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa; gram-positive bacteria Bacillus subtilis; Staphylococcus aureus and Micrococcus luteus. The antifungal activity was estimated with the fungi of the genus Aspergillus (A. niger, A. fumigatus) and the yeast Candida albicans. Amphotericin B, nystatin, and amoxiclav were used as a positive control. An activity of the ND against E. coli, B. subtilis and S. aureus was observed. As for fungi, the ND effect was detected for all the test strains including yeast. Interestingly that ND can combine antibacterial and antifungal properties. The antimicrobial action of nanoparticles is still poorly understood [1]. In the presented work using of luminescence spectroscopy and fluorescence lifetime imaging, Raman spectroscopy with utilizing of the ND’s optical-spectroscopic properties is discussed for the study of the ND interaction with the bacteria and fungi.