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The development of compounds for measuring of distribution and concentration of biologically important metal ions, e.g. zinc, displays a particular interest for sensor technology. It was shown that the presence of two coordinating sites in 2-styrylphenanthroline, which contains crown ether moiety, leads to the formation of supramolecular assembles of different structure. The binding of metal cations with different coordinating sites of the ligand resulted in different optical responses.