ИСТИНА

Cardiovascular diseases are the main cause of death in the world. Therefore, search and development of new therapeutic agents are significant. The application of micromycetes as producers of fibrinolytic agents has great potential. In this work, fibrinolytic activity of Aspergillus candidus 305 has been studied. It was found that the optimum conditions for growth and protease production were the highest at pH 6.0 and temperature 37°С. The maximum plasmin-like activity of A.candidus was observed after submerged cultivation for 96 hours, reaching 72,674 U/ml×10−3. Additionally, fibrinolytic activity was detected using fibrin plates (584,415 AU/ml). After isoelectric focusing a protease with pI 3,44 – 4,47 was detected. Isolated enzyme had high plasmin-like and thrombin-like activity determined with chromogenic peptide substrates – 38,86 E/ml and 44,254 E/ml, respectively. The molecular weight of the protease of A. candidus 305 - 35 kDа was determined by SDS-PAGE. Dot blot method and PAS-reaction showed that protease of A. candidus 305 is not glycosylated. The inhibitory analysis indicated that the protease belongs to serine class. Thus, represented data show the potential of A. candidus 305 and its application in thrombolytic therapy.