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The territory of museum - reserve “Kolomenskoye” is a landscape-architectural complex that includes the world-famous monuments. Natural Foundation has the same cultural-historical value as an architectural ensemble. In “Kolomenskoye” grows many age-related plants. Here is preserved the trees of English oak (Quercus robur), planted in the 16th century, fragments of the landscape Park "Lipki" and the avenue of limes, planted in the early 19th century, the city's only grove of European ash (Fráxinus excelsior), well known in Moscow gardens of Kolomenskoye. The proximity of the big city creates a huge recreational load, therefore the pollution of air, soil arises. The conditions of the flora existence and development are deteriorated. One of the major aims in the environmental activities of the museum – reserve is the maintIn this regard, the objectives of the study were to assess the anthropogenic load on natural objects on the territory of the reserve depending on distance from the highway. To fulfill environmental studies the method of snow and soil probes sampling was developed. The samples were analyzed for heavy metals (HM) content. enance, preservation and restoration of the natural environment.