ИСТИНА

The cellular composition of the aortic intima is extremely diverse. It is the cells of this layer that are the first to encounter damaging factors and react to their effects. Loss of ability to withstand negative influences leads to the development of various vascular diseases. In addition, initial genetic features can affect cell function, which may be the cause of the development of an aortic aneurysm. The aim of our work was to study various cell populations of the primary cell culture of the subendothelial layer of the human aortic intima, the ability of these cells to phagocytosis and LDL uptake, and their proliferative potential. The primary cells were isolated from human aortic samples, which were collected in accordance with the principles of medical ethics. The obtained samples were treated with a cocktail of proteolytic enzymes. In the course of the work, experiments were carried out to study the phagocytic activity of cells by co-incubation with atherogenic LDL and latex beads. The isolated cells were subjected to an immunophenotyping procedure. Immunofluorescence study showed that from 15 to 30% of single-passage cells isolated from the subendothelial layer of the aortic intima carry pericytic and MSC markers: CD146, CD73, CD105, CD13, NG2, CD140b. In the analysis of freshly isolated cells without passing, the number of cells with such a set of markers was up to 5%. Analysis of the ability to absorb latex beads and LDL showed that these functions of these cells are comparable to the properties of professional human phagocytes. It was also found that in comparison with cells isolated from the human aorta without an aneurysm, the cells of the intima of the aneurysmal aorta are able to multiply for a long time in culture up to 20 passages. While conditionally normal cells stop proliferating after 5 passages. Our data indicates that the cells of the subendothelial layer are in dynamic equilibrium, which can change under the influence of damaging factors and lead to the formation of pathological changes in the form of various lesions. Our further work will consist in the specific sorting and immortalization of isolated cells. This will make it possible to obtain a cell model for studying the processes that initiate the development of aneurysms and other vascular changes, such as atherosclerosis. Funding Source: This work was supported by the Russian Science Foundation (Petrovsky National Research Center of Surgery Grant No22-65-00089). Keywords: aneurysm, pericytes, cell proliferation