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The new mycroscopy method for Native Assessment of Sperm Ultramorphology (NASUM) was developed. NASUM gives possibility to visualize chromatin, acrosome and its granularity, vacuoles, mitochondria, nuclear membrane pores, microfibrils of the tail, and chromocenter, as well as evaluate the degree of chromatin condensation in native spermatozoa. The aim of this study was developing a new microscopy method of sperm morphology evaluation, allowing us to visualize the intracellular organelles of spermatozoa.