ИСТИНА

The most notable example of the 3D genome organization influence on transcription is the gene expression switching in multigenic tissue-specific geneтclusters. One of them is the keratin gene cluster which is located in chromosome 12 (12q13.13) and contains 26 genes. Potential locus control regions (LCRs) that we have previously discovered could be “switchers” of gene expression and transcriptional regulators of entire multigenic locus. Thus, the aim was to study the spatial structure of the keratin gene locus in mature keratinocytes as well as during the differentiation, including the analysis of LCR contribution in the formation of the locus topology. We also analyzed the mechanisms involved in the formation of the spatial configurationusing drug treatments