ИСТИНА

In this work, the intercellular interaction of erythrocytes and endothelial cells was studied in vitro using optical methods. Using laser tweezers, the adhesion forces of erythrocytes to the endothelium were measured, as well as the aggregation forces of erythrocytes upon addition of various compounds (L-Arginine, L-NAME) affecting the interaction of these cells to blood plasma or whole blood. The aggregation of erythrocytes in the blood flow in the presence of endothelial cells was studied using the laser aggregometry technique. The results demonstrate a decrease in the aggregation forces of erythrocytes in the presence of the endothelium upon stimulation of nitric oxide release. In the flow, erythrocyte aggregation also decreases in the presence of endothelial cells. The adhesion forces of erythrocytes to the endothelium do not change or decrease slightly (in some cases) with an increase in the concentration of nitric oxide. This work was supported by the Russian Science Foundation (Grant No. 23-45-00027).