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Several optical techniques were used to make a complex study of various parameters related to the fundamental properties of erythrocytes, aggregation and deformation, by means of imaging and measurement. Diffuse light scattering, laser diffractometry, and dual- and multiple channel laser tweezers were applied in the experiments in vitro. Computerized capillaroscopy and laser two-photon lifetime imaging microscopy were used for imaging the structure of the terminal capillaries and papillary dermis in the fingernail bed and the inner forearm area in vivo.