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Cross-linking as a tool for enhancement of transfection efficiency of cationic vectorsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 19 сентября 2015 г.
- Авторы: Maximova Ekaterina D., Faizuloev Evgeny B., Nikonova Alexandra A., Kotova Svetlana L., Solov’eva Anna B., Izumrudov Vladimir A., Litmanovich Ekaterina A., Kudryashova Elena V., Melik-Nubarov Nickolay S.
- Журнал: European Polymer Journal
- Том: 69
- Год издания: 2015
- Издательство: Pergamon Press Ltd.
- Местоположение издательства: United Kingdom
- Первая страница: 110
- Последняя страница: 120
- DOI: 10.1016/j.eurpolymj.2015.05.024
- Аннотация: A series of nanogel particles with the growing cross-linking degree was synthesized in reverse micelles by copolymerization of a cationic monomer N,N-dimethylaminoethylme thacrylate with increasing amounts of N,N0-methylenebisacrylamide (MBA). The growth of MBA content in the studied range of 0.2–15% (mol) resulted in a regular decrease of the particles’ size with a narrowing in their size distribution which occurred at the first of the cross-linker addition. In the second region, where the MBA content was more than 5% (mol), the size distribution was very narrow. The cross-linked polymers prepared at 2–5% of the cross-linker amount demonstrated a minimum size of the polyplexes and enhancement in the transfection with plasmid DNA and small interfering RNA targeted to the luciferase gene. The revealed changes in the properties of nanogels occurring upon the changes in the cross-linking degree appear to be a platform for a controlled manufacturing of efficient cross-linked cationic vectors.
- Добавил в систему: Кудряшова Елена Вадимовна