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Microvasculature on a chip: study of the Endothelial Surface Layer and the flow structure of Red Blood Cellsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 апреля 2018 г.
- Авторы: Tsvirkun Daria, Grichine Alexei, Duperray Alain, Misbah Chaouqi, Bureau Lionel
- Журнал: Scientific reports
- Том: 7
- Номер: 45036
- Год издания: 2017
- Издательство: Nature Publishing Group
- Местоположение издательства: United Kingdom
- Первая страница: 1
- Последняя страница: 11
- DOI: 10.1038/srep45036
- Аннотация: Microvasculatures-on-a-chip, i.e. in vitro models that mimic important features of microvessel networks, have gained increasing interest in recent years. Such devices have allowed investigating pathophysiological situations involving abnormal biophysical interactions between blood cells and vessel walls. Still, a central question remains regarding the presence, in such biomimetic systems, of the endothelial glycocalyx. The latter is a glycosaminoglycans-rich surface layer exposed to blood flow, which plays a crucial role in regulating the interactions between circulating cells and the endothelium. Here, we use confocal microscopy to characterize the layer expressed by endothelial cells cultured in microfluidic channels. We show that, under our culture conditions, endothelial cells form a confluent layer on all the walls of the circuit and display a glycocalyx that fully lines the lumen of the microchannels. Moreover, the thickness of this surface layer is found to be on the order of 600 nm, which compares well with measurements performed ex or in vivo on microcapillaries. Furthermore, we investigate how the presence of endothelial cells in the microchannels affects their hydrodynamic resistance and the near-wall motion of red blood cells. Our study thus provides an important insight into the physiological relevance of in vitro microvasculatures.
- Добавил в систему: Цвиркун Дарья Викторовна