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Development of a chemiluminescent enzyme immunoassay for the determination of dexamethasone in milkстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Vdovenko Marina M., Gribas Anastasia V., Vylegzhanina Alexandra V., Sakharov Ivan Yu
- Журнал: Analytical Methods
- Том: 4
- Год издания: 2012
- Издательство: Royal Society of Chemistry
- Местоположение издательства: United Kingdom
- Первая страница: 2550
- Последняя страница: 2554
- DOI: 10.1039/c2ay25278c
- Аннотация: An indirect competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) for the determination of dexamethasone (DEX) was developed using soybean peroxidase (SbP) as an enzyme label. A mixture of 3-(100-phenothiazinyl)-propane-1-sulfonate (SPTZ) and 4-morpholinopyridine (MORPH) was used as an enhancer of SbP-induced chemiluminescence. Varying the concentrations of the capture antigen (DEX-ovalbumin) and specific anti-DEX antibody, the conditions of the assay were optimized. The values of IC10, IC50 and working range (IC20–IC80) of the CL-ELISA of DEX were 0.02, 0.9, 0.08–9.3 ng mL1, respectively. It was shown that a pretreatment of cow milk samples by centrifugation and 25% methanol prevented the matrix effect of whole milk. The coefficient of variation (CV) and recovery values from the spiked milk samples estimated by the developed CL-ELISA were in the range of 2.2 to 9.9% and 82 to 142%, respectively.
- Добавил в систему: Сахаров Иван Юрьевич