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Human and yeast DNA damage recognition complexes bind with high affinity DNA structures mimicking in size transcription bubbleстатья Исследовательская статья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 17 апреля 2018 г.
- Авторы: Krasikova Yuliya S., Rechkunova Nadejda I., Maltseva Ekaterina A., Anarbaev Rashid O., Pestryakov Pavel E., Sugasawa Kaoru, Min Jung-Hyun, Lavrik Olga I.
- Журнал: Journal of Molecular Recognition
- Том: 26
- Номер: 12
- Год издания: 2013
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: 653
- Последняя страница: 661
- DOI: 10.1002/jmr.2308
- Аннотация: The human XPC-RAD23B complex and its yeast ortholog, Rad4-Rad23, are the primary initiators of global genome nucleotide excision repair. In this study, two types of DNA binding assays were used for the detailed analysis of interaction of these proteins with damaged DNA. An electrophoretic mobility shift assay revealed that human and yeast orthologs behave similarly in DNA binding. Quantitative analyses of XPC/Rad4 binding to the model DNA structures were performed using fluorescent depolarization measurements. The XPC-RAD23B and the Rad4-Rad23 proteins bind to the damaged 15nt bubble-DNA structure mimicking in size the transcription bubble DNA intermediate with the highest affinity (K-D values 10(-10)M or less) that is reduced in the following order: damaged bubble>undamaged bubble>damaged duplex>undamaged duplex. The affinity of XPC/Rad4 for various DNAs was shown to correlate with DNA bending angle. The results obtained show clearly that more deviation from regular DNA structure leads to higher XPC/Rad4 affinity. Copyright (c) 2013 John Wiley & Sons, Ltd.
- Добавил в систему: Пестряков Павел Ефимович