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The outward component of photoinduced current in chloroplasts of Peperomia metallicaстатья
Информация о цитировании статьи получена из
Scopus
Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.
- Авторы: Cherkashin A.A., Bulychev A.A., Vredenberg W.J.
- Журнал: Membrane and Cell Biology
- Том: 14
- Номер: 4
- Год издания: 2001
- Первая страница: 475
- Последняя страница: 485
- Аннотация: The photoinduced currents in whole chloroplasts of Peperomia metallica were studied using suction electrodes and single-turnover flashes. The kinetic profile of the photocurrent contained a minor outward component (rise time, 100 µs). Local application (from the inside of the pipette) of a photosystem 2 inhibitor, DCMU, rapidly suppressed the outward current; conversely, the addition of DCMU to the outer medium produced a transient stimulation of the outward component. Permeabilization of the tip-located membrane fragments with Triton X-100 eliminated the outward current, but had no significant influence on the inward current. The data suggest that the outward current originated in the tip-located nonruptured portions of the thylakoid membrane. Different involvement of two photosystems in the generation of the outward current indicates that granal thylakoids enriched with photosystem 2 are less susceptible to the rupture in the pipette tip as compared with stromal thylakoids.
- Добавил в систему: Булычев Александр Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Статья в Membr. Cell Biol. "Фототок в хлоропластах" | 4-03_Cherkashin_475-485.pdf | 215,7 КБ | 25 августа 2017 [bulychev] |