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Combination of the electrogenic ionophores, valinomycin and CCCP, can lead to non-electrogenic K+/H+ exchange on bilayer lipid membranesстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
- Авторы: Orlov Victor N., Antonenko Yuri N., Bulychev Alexander A., Yaguzhinsky Lev S.
- Журнал: FEBS Letters
- Том: 345
- Номер: 2–3
- Год издания: 1994
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 104
- Последняя страница: 106
- DOI: 10.1016/0014-5793(94)00420-X
- Аннотация: The method of pH shift measuring by means of a pH microelectrode was applied to measure hydrogen ion fluxes across a planar bilayer lipid membrane (BLM) in the presence of the potassium ion ionophore, valinomycin, and a protonophore, carbonylcyanide m-chlorophenylhydrazone (CCCP), under conditions of the voltage clamp. The voltage dependence of the flux was determined to be in the range of ± 150 mV under the conditions of both symmetrical KCl as well as a KCl gradient across the BLM. Surprisingly, at a clamped zero voltage on BLM a significant hydrogen ion flux was observed in the presence of a KCL gradient and both valinomycin and CCCP. This finding was interpreted as a result of induction of non-electrogenic K+/H+ exchange in the presence of valinomycin and CCCP, presumably through the formation of electrically neutral complexes of these two ionophores and K+ (H+) ions: valinomycin—K+—CCCP− and/or possibly valinomycin—CCCP−—H+.
- Добавил в систему: Булычев Александр Александрович