Crosslinking of (cytosine-5)-DNA methyltransferase SsoII and its complexes with specific DNA duplexes provides an insight into their structures

Ryazanova, A.Y.; Winkler, I.; Friedhoff, P.; Viryasov, M.B.; Oretskaya, T.S.; Kubareva, E.A.

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.

Авторы: Ryazanova A.Yu, Winkler I., Friedhoff P., Viryasov M.B., Oretskaya T.S., Kubareva E.A.
Журнал: Nucleosides, Nucleotides and Nucleic Acids
Том: 30
Номер: 7-8
Год издания: 2011
Издательство: Marcel Dekker Inc.
Местоположение издательства: United States
Первая страница: 632
Последняя страница: 650
DOI: 10.1080/15257770.2011.584339
Аннотация: (Cytosine-5)-DNA methyltransferase SsoII (M.SsoII) functions as a methyltransferase and also as a transcription factor. Chemical and photochemical crosslinking was used for exploring the structure of M.SsoII–DNA complexes and M.SsoII in the absence of DNA. Photocrosslinking with 4-(N-maleimido)benzophenone demonstrated that in the M.SsoII complex with DNA containing the regulatory site, the M.SsoII region responsible for methylation was bound to DNA flanking the regulatory site, which contained no methylation sequence. This required high flexibility of the linker connecting the M.SsoII N-terminal domain and the M.SsoII region responsible for methylation. The flexibility was demonstrated by crosslinking with bis-maleimidoethane and 1,11-bis-maleimidotetraethyleneglycol.
Добавил в систему: Рязанова Александра Юрьевна

	ИСТИНА	Войти в систему Регистрация
	ИСТИНА ЦЭМИ РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА

ИСТИНА ЦЭМИ РАН

Crosslinking of (cytosine-5)-DNA methyltransferase SsoII and its complexes with specific DNA duplexes provides an insight into their structuresстатья

Прикрепленные файлы