|
ИСТИНА |
Войти в систему Регистрация |
ИСТИНА ЦЭМИ РАН |
||
Disruption of Amyloid Prion Protein Aggregates by Cationic Pyridylphenylene Dendrimersстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Sorokina Svetlana A., Stroylova Yulia Yu, Shifrina Zinaida B., Muronetz Vladimir I.
- Журнал: Macromolecular Bioscience
- Год издания: 2016
- Издательство: John Wiley & Sons Ltd.
- Местоположение издательства: United Kingdom
- DOI: 10.1002/mabi.201500268.
- Аннотация: Disruption of amyloid protein aggregates is one of the potential therapies for treatment of neurodegenerative disorders such as prion diseases. Here, for the first time we report that pH-independent cationic pyridylphenylene dendrimers are able to disrupt amyloid protein aggregates at physiological pH as exemplified by inclusion bodies of ovine prion protein. The results show that exposure of inclusion bodies to the dendrimers leads to its partial disaggregation and release of the nanosize protein-dendrimer complexes. The complexes were characterized by SDS PAGE, DLS, and Western blotting methods. Thioflavin T fluorescence clearly demonstrated a decrease of amyloidogenic capability of the prion protein upon exposure to the dendrimers. The complexes formed are stable and do not show further aggregation.
- Добавил в систему: Муронец Владимир Израилевич