Atomic force microscopy of living and fixed Xenopus laevis embryos

Efremov, Y.M.; Pukhlyakova, E.A.; Bagrov, D.V.; Shaitan, K.V.

Информация о цитировании статьи получена из Web of Science, Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.

Авторы: Efremov Yu M., Pukhlyakova E.A., Bagrov D.V., Shaitan K.V.
Журнал: Micron
Том: 42
Номер: 8
Год издания: 2011
Издательство: Pergamon Press Ltd.
Местоположение издательства: United Kingdom
Первая страница: 840
Последняя страница: 852
DOI: 10.1016/j.micron.2011.05.010
Аннотация: Xenopus laevis embryos are a rather simple and at the same time a very interesting animal model, which is widely used for research in developmental biology. Intensive coordinated cell movements take place during the multi-cellular organism development. Little is known of the cellular, molecular and biomechanical mechanisms of these movements. The conceptual framework for analysis of cell interactions within integrated populations is poorly developed. We have used atomic force microscopy (AFM) to observe the surface of fixed X. laevis embryos at different stages of their development. We have developed a new sample preparation protocol for these observations. The obtained images were compared with scanning electronic microscopy (SEM) data. Cell rearrangement during morphogenesis in vivo was also visualized by AFM. In the current paper we discuss facilities and challenges of using this technique for further embryo researching.
Добавил в систему: Багров Дмитрий Владимирович

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Atomic force microscopy of living and fixed Xenopus laevis embryosстатья

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