Association of polymorphisms of genes of NO synthases and migraine in Moscow

Kondratieva, N.S.; Azimova, J.E.; Sergeev, A.; Skorobogatykh, K.; Fokina, N.M.; Kokaeva, Z.G.; Tabeeva, G.R.; Klimov, E.A.

Авторы: Kondratieva N.S., Azimova J.E., Sergeev A., Skorobogatykh K., Fokina N.M., Kokaeva Z.G., Tabeeva G.R., Klimov E.A.
Сборник: Abstracts for IHC2015
Серия: Cephalalgia: an international journal of headache
Том: 35
Год издания: 2015
Издательство: SAGE Publications
Местоположение издательства: United States
Первая страница: 266
Последняя страница: 266
DOI: 10.1177/0333102415581304
Аннотация: Background: Nitric oxide (NO) is an important mediator of vasodilation in the intra- and extracranial vessels. This small molecule easily penetrates the membranes of vascular smooth muscle cell, and activates soluble guanylate cyclase and cGMP synthesis, leading to vasodilation. NO also promotes the release of CGRP, which is a powerful vasodilator. The SNPs in genes, coding NO synthases, may influence the pathogenesis of migraine. The aim of the study was to investigate the prevalence of iNOS (rs2779249), eNOS (rs2070744) and nNOS (rs41279104) polymorphisms of the NOS family genes in migraine patients and controls. Method: 146 migraine patients (44 with chronic and 96 with episodic migraine, ICHD-III), and 363 unscreened volunteers as the controls (all live in Moscow). SNPs were genotyped by RT-PCR technique using qPCRmix-HS kit (Evrogen) and oligos synthesized by DNA Synthesis, LLC. Reactions were performed in CFX-96 software (BioRad). Results: Alleles and genotypes frequencies of SNPs in iNOS (rs2779249) and nNOS (rs41279104) were not different in samples of patients and controls. For the SNP in eNOS gene (rs2070744) an association of GG genotype with migraine was found (Chi-square 4.47, p=0.03, OR=1.88 95%CI=[1.09–3.24], Cochran-Armitage test), the G allele is recessive allele (Chi-square 5.22, p=0.02, OR=1.88, 95%CI=[1.09–3.24]). When comparing the groups of CM and EM with control the association only with EM was found (Chi-square 4.07, p=0.04, OR=2.03, 95%CI=[1.09–3.76], Cochran-Armitage test). Conclusion: The influence of the recessive G allele (rs2070744) of eNOS gene on the pathogenesis of episodic migraine was shown. No conflict of interest. WOS:000354559400524 DOI: 10.13140/RG.2.1.1478.3761
Добавил в систему: Климов Евгений Александрович

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Association of polymorphisms of genes of NO synthases and migraine in Moscowстатья

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