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Monitoring of blood oxygenation in brain by resonance Raman spectroscopyстатья
Статья опубликована в высокорейтинговом журнале
Статья опубликована в журнале из списка Web of Science и/или Scopus- Авторы: Brazhe Nadezda A., Kirsten Thomsen, Micael Lønstrup, Brazhe Alexey R., Nikelshparg Evelina I., Maksimov Georgy V., Olga Sosnovtseva Martin Lauritzen
- Журнал: Journal of Biophotonics
- Год издания: 2018
- Издательство: Wiley - VCH Verlag GmbH & CO. KGaA
- Местоположение издательства: Germany
- DOI: 10.1002/jbio.201700311
- Аннотация: Blood oxygenation in cerebral vessels is an essential parameter to evaluate brain function and to investigate the coupling between local blood flow and neuronal activity. We apply resonance Raman spectroscopy in vivo to study hemoglobin oxygenation in cortex vessels of anesthetized ventilated mice. We demonstrate that the pairs of Raman peaks at 1355 and1375 cm-1 (symmetric vibrations of pyrrol half-rings in the heme molecule), 1552 and 1585 cm-1 and 1602 and 1638 cm-1 (vibrations of methine bridges in heme molecule) are reliable markers for quantitative estimation of the relative amount of oxyhemoglobin in venules, arterioles, and capillaries. in vivo measurements of blood oxygenation in the cortex of mice ventilated with inspiratory gas mixtures containing different amounts of oxygen-normoxia, hyperoxia and hypoxia-validate the proposed approach. Our method allows to visualize blood saturation with O2 in different microvascular networks.
- Добавил в систему: Браже Надежда Александровна