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Activation of TLR3 and TLR4 in long- and short-term cells incubation of astrocytes in high-glucose mediumстатья Тезисы
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 16 января 2019 г.
- Авторы: Azbukina N., Chistiakov D., Astakhova A., Sergeeva M.
- Журнал: FEBS open bio
- Том: 8
- Номер: S1
- Год издания: 2018
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: 380
- Последняя страница: 380
- DOI: 10.1002/2211-5463.12453
- Аннотация: Bacterial and viral agents have different effects on the inflammatory responses in hyperglycemia. Accumulating studies have shown that hyperglycemia show inflammation in the central nervous system (CNS). Astrocytes play an important role in the development of inflammation responses in CNS. However, changes in inflammatory responses at different glucose concentrations are not well characterized. The aim of our research was to investigate the influence of long- and short-term high glucose (HG) cultivation medium on the response of astroglial cells to inflammatory stimuli. We compare inflammatory responses in course of the activation of Toll-like receptors (TLR) with a bacterial-like (TLR4) and viral-like (TLR3) stimuli. Primary astrocytes from newborn rats were obtained from brain cortices by conventional procedures. Cells were cultured 12 days before experiments in either DMEM with 5 mM glucose (normal glucose level, NG) or in media with 22.5 mM glucose (long-term high glucose level). In short-term HG model cells, 5 mM glucose DMEM was switched to 22.5 mM glucose for 48 h before stimulations. Astrocytes were stimulated with the lipopolysaccharide (LPS, a TLR4 agonist) or the poly I:C (PIC, a TLR3 agonist). Analysis of mRNA expression of cyclooxygenase 2 (COX-2), TNF-alpha and IL-10 was performed by qPCR, protein levels of COX-2 were determined by Western blot, levels of released cytokines IL-10, TNF-alpha and prostaglandin E2 (PGE2) were determined using ELISA kits. We obtained that inflammatory response to LPS reduced in long-term HG and induced in short-term HG (in comparison with NG). Both types of HG treatments induced inflammatory response to PIC. Effects were observed on mRNA levels of IL-10, TNF-alpha and both protein and mRNA of COX-2. Alterations in concentrations of released mediators IL-10, TNF-alpha and PGE2 were detected. The difference between PIC and LPS action was observed in a time-dependence manner. Supported by RSF (№ 16-15-10298).
- Добавил в систему: Чистяков Дмитрий Викторович