Unified strategy for HPLC-MS evaluation of bioactive compounds for quality control of herbal products

Turova, P.; Stekolshchikova, E.; Baygildiev, T.; Shpigun, O.; Rodin, I.; Stavrianidi, A.

Авторы: Polina Turova, Elena Stekolshchikova, Timur Baygildiev, Oleg Shpigun, Igor Rodin, Andrey Stavrianidi
Журнал: Biomedical Chromatography
Том: 32
Номер: 12
Год издания: 2018
Издательство: John Wiley & Sons Inc.
Местоположение издательства: United States
Первая страница: e4363
DOI: 10.1002/bmc.4363
Аннотация: Evaluation of safety, quality and composition of herbal products and food supplements based on botanical ingredients is a matter of serious concern. For screening of botanicals in herbal products multi-targeted and group-targeted approaches may be applied. In the group-targeted approach botanicals are characterized by means of appropriate group of structurally related biomarkers instead of multi-targeted approach where the number of selected analytes are monitored based on multiple reaction monitoring survey. In this study unified strategy for quality control of herbal products was developed on the basis of fast ultrasound assisted extraction, chromatographic separation and mass spectrometric quantification of bioactive compounds. A large list of unique biomarkers were monitored under almost identical chromatographic conditions, while also an efficient strategy for HPLC-MS group-targeted analysis was developed for comprehensive evaluation of chemical composition of botanicals intensively used for herbal product manufacturing. In the later case, structurally close compounds were determined in a single ion monitoring mode for the characteristic group of fragment ions, allowing fast profiling and quality assessment of the plant material or complex food supplement. The sensitivity of the developed approaches was on the level of 1-50 ng/mL which is higher than of existing HPLC-UV quality control methods.
Добавил в систему: Ставрианиди Андрей Николаевич

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