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Electrochemical immunosensor for Forest-Spring encephalitis based on protein a labeled with colloidal gold /Brainina K., Kositzina A., Beikin, J. // Analytical and Bioanalytical Chemistry. – 2003. – V. 376. – P. 481 – 485статья
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Дата последнего поиска статьи во внешних источниках: 30 августа 2018 г.
- Авторы: Brainina, K, Kozitsina, Beikin, J
- Журнал: Analytical and Bioanalytical Chemistry
- Том: 376
- Номер: 4
- Год издания: 2003
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 481
- Последняя страница: 485
- DOI: 10.1007/s00216-003-1912-3
- Аннотация: An electrochemical immunosensor for diagnosis of Forest-Spring encephalitis has been proposed. It comprises a screen-printed thick-film graphite electrode serving as the transducer and a layer of the Forest-Spring encephalitis antigen immobilized on the electrode and functioning as the biorecognition substance. The procedure includes formation of an antigen-antibody immune complex, localization of colloidal gold-labeled protein A on the complex, and recording of gold oxidation voltammogram, which provides information about the presence and the concentration of antibodies in blood serum. The response is proportional to the concentration of antibodies over the interval from 10-7 to 10-2 mg mL-1. The detection limit is 10-7 mg mL-1.
- Добавил в систему: Козицина Алиса Николаевна