Fluorescence Polarization Immunoassay for Alternaria Mycotoxin Tenuazonic Acid Detection and Molecular Modeling Studies of Antibody Recognition

Wang, F.; Cai, J.; Eremin, S.A.; Xiao, Z.; Shen, Y.; Tian, Y.; Zhen-Lin, X.; Yang, J.; Lei, H.; Sun, Y.; Wang, H.

Авторы: Wang Feng, Cai Jun, Eremin Sergei A., Xiao Zhi-Li, Shen Yu-Dong, Tian Yuan-Xin, Zhen-Lin Xu, Yang Jin-Yi, Lei Hong-Tao, Sun Yuan-Ming, Wang Hong
Журнал: Food Analytical Methods
Том: 11
Номер: 9
Год издания: 2018
Издательство: Springer Pub. Co.,
Местоположение издательства: United States
Первая страница: 2455
Последняя страница: 2462
DOI: 10.1007/s12161-018-1236-8
Аннотация: A simple and rapid method of fluorescence polarization immunoassay (FPIA) for detection of Alternaria mycotoxin tenuazonic acid (TeA) was described. In this study, the tracers TeAH-FITC and TeAH-DTAF were synthesized from hapten 5-(sec-butyl)-3-(1-hydrazonoethyl)-4-hydroxy-1H-pyrrol-2(5H)-one (TeAH) and different fluoresceins isothiocyanate isomer I (FITC) or 5-([4,6-dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF), respectively. The established FPIA showed an IC50 value of 3.0 ?g/mL with a detection limit of 0.13 ?g/mL for TeAH based on the tracer of TeAH-FITC, and with the same properties of 7.3 and 0.93 ?g/mL based on TeAH-DTAF. The former gave better assay sensitivity than the latter, that the 2,4-dichloro-s-triazine group of TeAH-DTAF may affect the specific recognition of tracer and antibody. Models of the lowest energy conformation and the molecular electrostatic potential isosurface studies revealed that the –C=N–N– and –CHCH3(CH2CH3) groups were the characteristic chemical groups in anti-TeAH antibody-analyte recognition via molecular modeling, which could create a theoretical guide for hapten design of small molecules and provide the traditional theory with empirical proof.
Добавил в систему: Еремин Сергей Александрович

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Fluorescence Polarization Immunoassay for Alternaria Mycotoxin Tenuazonic Acid Detection and Molecular Modeling Studies of Antibody Recognitionстатья

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