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Contrasting of biological samples for X-ray synchrotron microtomographyстатья
- Авторы: Efimova OI, Khlebnikov AS, Senin RA, Voronin PA, Anokhin KV
- Журнал: Bulletin of Experimental Biology and Medicine
- Номер: Aug
- Год издания: 2013
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- DOI: 10.1007/s10517-013-2165-6
- Аннотация: The method of contrasting with iodine ions was developed to obtain high-resolution 3D images of large biological specimens using a synchrotron X-ray microtomography unit. It was shown that the samples (late mouse embryos) treated with 50% Lugol solution with addition of 25% ethanol for 48 h followed by a 48-h washout in phosphate buffered saline had maximum contrast and lowest compression artifacts. Processing of samples by this protocol allowed detecting zones of active proliferation. Incubation of brain samples for 120 h in 7.6% meglumine/sodium diatrizoate without washout ensured the best contrast during myelin identification.
- Добавил в систему: Анохин Константин Владимирович