Isolation and Characterization of the Water-Soluble Chromoproteins of Cyanobacteria Arthrospira platensis: C-phycocyanin, Allophycocyanin, Carotinoid- and Chlorophyll a-Binding Proteins

Telegina, T.A.; Biryukov, M.V.; Terekhova, I.V.; Vechtomova, Y.L.; Kritsky, M.S.

Авторы: Telegina T.A., Biryukov M.V., Terekhova I.V., Vechtomova Yu L., Kritsky M.S.
Журнал: Applied Biochemistry and Microbiology
Том: 54
Номер: 6
Год издания: 2018
Издательство: Maik Nauka/Interperiodica Publishing
Местоположение издательства: Russian Federation
Первая страница: 631
Последняя страница: 638
DOI: 10.1134/S0003683818060145
Аннотация: A method was developed for the simultaneous isolation of water-soluble chromoproteins from cyanobacteria Arthrospira platensis: C-phycocyanin, allophycocyanin, carotenoid- and chlorophyll-binding proteins by chromatography on DEAE-Toyopearl 650M. The purity of the isolated C-phycocyanin was 4.42 (A620/A280). Allophycocyanin showed the purity of 3.40 (A652/A280). The purity of phycocyanin was confirmed by the presence of single bands of the α-subunit (17 kDa) and the β-subunit (18 kDa) during SDS-PAGE. The isolated carotenoid- and chlorophyll-binding proteins were analyzed by high-performance exclusion chromatography on TSK-GEL 2000SW (XL) with detection at three wavelengths (280, 480 and 678 nm). The spectral, chromatographic and electrophoretic analyzes of chromoproteins, as well as the analysis of pigments allowed us to conclude that the carotenoid-chlorophyll a-binding protein is a xanthophyll-chlorophyll a-protein complex, and chlorophyll a-binding protein is a chlorophyll a-protein complex. The molecular masses of the chlorophyll a-binding protein and carotenoid-chlorophyll a-binding protein evaluated by high-performance exclusion chromatography and SDS-PAGE were 57 and 16 kDa, respectively. Photoprotective properties of these proteins and their possible functioning as part of evolutionary precursors of photosynthetic systems are discussed. Keywords: spirulina, Arthrospira platensis, allophycocyanin, C-phycocyanin, water-soluble carotinoid-chlorophyll a-binding protein, water-soluble chlorophyll a-binding protein, evolution of photosynthesis
Добавил в систему: Бирюков Михаил Владимирович

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Isolation and Characterization of the Water-Soluble Chromoproteins of Cyanobacteria Arthrospira platensis: C-phycocyanin, Allophycocyanin, Carotinoid- and Chlorophyll a-Binding Proteinsстатья

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