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Chemical ligation of DNA: the first non-enzymatic assembly of a biologically active geneстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Shabarova Z.A., Merenkova I.N., Oretskaya T.S., Sokolova N.I., Skripkin E.A., Alexeyeva E.V., Balakin A.G., Bogdanov A.A.
- Журнал: Nucleic Acids Research
- Том: 19
- Номер: 15
- Год издания: 1991
- Издательство: Oxford University Press
- Местоположение издательства: United Kingdom
- Первая страница: 4247
- Последняя страница: 4251
- Аннотация: An artificial gene comprising 183 base pairs has been assembled by template-directed condensation of 35- to 53-membered oligodeoxyribo nucleotides with cyanogen bromide as a condensing agent. The reaction is complete within several minutes at 0δC in buffer. The resulting mini-gene was cloned and expressed in vivo and in vitro. We have also found that the polymerase inhibition technique (toe-printing) is a good way to ascertain that translation initiation complexes form in the case of single-stranded DNAs as well. Thus, along with the fully chemical assembly of synthetic genes, a rapid and sufficiently reliable method for determining their ribosome-binding properties was developed.
- Добавил в систему: Орецкая Татьяна Семеновна