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DNA interactions of dinuclear RuII arene antitumor complexes in cell-free mediaстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Nováková O., Nazarov A.A., Hartinger C.G., Keppler B.K., Brabec V.
- Журнал: Biochemical Pharmacology
- Том: 77
- Номер: 3
- Год издания: 2009
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 364
- Последняя страница: 374
- DOI: 10.1016/j.bcp.2008.10.021
- Аннотация: We recently synthesized and characterized water-soluble dinuclear RuII arene complexes, in which two (η6-p-isopropyltoluene)RuCl[3-(oxo-κO)-2-methyl-4-pyridinonato-κO4] units were linked by flexible chains of different length [(CH2)n (n = 4, 6, 8, 12)]. These new dinuclear ruthenium drugs were found to exert promising cytotoxic effects in human cancer cells. In the present work DNA modifications by these new dinuclear RuII arene compounds, which differed in the length of the linker between the two RuII centers, were examined by biochemical and biophysical methods. The complexes bind DNA forming intrastrand and interstrand cross-links in one DNA molecule in the absence of proteins. An intriguing aspect of the DNA-binding mode of these dinuclear RuII compounds is that they can cross-link two DNA duplexes and also proteins to DNA-a feature not observed for other antitumor ruthenium complexes. Thus, the concept for the design of interhelical and DNA-protein cross-linking agents based on dinuclear RuII arene complexes with sufficiently long linkers between two Ru centers may result in new compounds which exhibit a variety of biological effects and can be also useful in nucleic acids research. © 2008 Elsevier Inc. All rights reserved.
- Добавил в систему: Назаров Алексей Анатольевич