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Marginal blebbing during the early stages of TNF-induced apoptosis indicates alteration in actomyosin contractilityстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Domnina L.V., Ivanova O.Y., Pletjushkina O.Y., Fetisova E.K., Chernyak B.V., Skulachev V.P., Vasiliev J.M.
- Журнал: Cell Biology International
- Том: 28
- Номер: 6
- Год издания: 2004
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 471
- Последняя страница: 475
- DOI: 10.1016/j.cellbi.2004.03.017
- Аннотация: Dynamics of alterations of cell surface topography during TNF-induced apoptosis of HeLa cells was examined by phase-contrast videomicroscopy and immunomorphological analysis. The final stage of apoptosis accompanied by cell rounding and general blebbing of the cell surface became after 4-6h of incubation but much earlier, after 1.5-3h, essentially flattened lamellipodia at the active edges transformed into the small blebs that were continuously extended and retracted during the next 1-2h. This phenomenon was called "marginal blebbing". It took place before the cytochrome c release from mitochondria to cytosol. Marginal blebbing was inhibited by drugs that depolymerized actin microfilaments (cytochalasin, latrunculin) or decreased Rho-kinase-dependent contractility of actin-myosin cortex (H7, HA-1077, Y27632). A pancaspase inhibitor, zVAD-fmk, completely prevented marginal and general blebbing, and TNF-induced apoptosis. DEVD-fmk, a specific inhibitor of caspase-3, inhibited both marginal and general blebbing but not cell rounding and death. Thus, marginal blebbing is an early microfilament-dependent apoptotic event. It is suggested that it is initiated by minimal activation of caspase-3 and the following local Rho-kinase-dependent stimulation of actin-myosin cortex contractility. Localization of marginal blebs at the active edge may be associated with special organization of cortex in that zone. © 2004 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
- Добавил в систему: Домнина Лидия Владимировна