Delayed luminescence of erythrosine in biological tissue and photodynamic therapy dosimetry

Letuta, S.N.; Pashkevich, S.N.; Ishemgulov, A.T.; Lantukh, Y.D.; Alidzhanov, E.K.; Sokabaeva, S.S.; Bryukhanov, V.V.

Авторы: Letuta S.N., Pashkevich S.N., Ishemgulov A.T., Lantukh Yu D., Alidzhanov E.K., Sokabaeva S.S., Bryukhanov V.V.
Журнал: Journal of Photochemistry and Photobiology B: Biology
Том: 163
Год издания: 2016
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 231
Последняя страница: 236
DOI: 10.1016/j.jphotobiol.2016.08.036
Аннотация: The features of delayed ﬂuorescence (DF) and phosphorescence in erythrosine stained healthy and cancer-diseased mammary gland tissues of BYRB-line mice were investigated in vitro. Thermoactivated delayed ﬂuorescence (TDF), triplet-triplet annihilation (TTA) and originated from singlet-triplet annihilation (STA) delayed ﬂuorescence are investigated as competing channels of radiative relaxation of the triplet states of erythrosine. The dominant role of diffusive-mobile molecular oxygen in deactivation of triplet-excited long-live states of the dye molecules in cells is determined. The previously not described phenomenon of light quenching of DF under pulsed laser irradiation (light quenching of DF, LQDF) in stained tissue was revealed. LQDF is increased if the energy of excited pulses is rise and their sequence period is decreased. The DF depletion disappears if time interval between pulses in the series is N5 s. This phenomenon is due two process competition: fast consumption of singlet oxygen by oxidation of cell organelles right after laser pulse excitation and slow diffusive recovery of oxygen concentration in the pause between the pulses. Statistically valid distinction between DF characterization as well as LQDF erythrosine extent in healthy and pathological tissues was established. The use of this phenomenon will greatly simplify the determination of radiation “dose” in photodynamic therapy (PDT) directly during the treatment session.
Добавил в систему: Ишемгулов Азамат Талгатович

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