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Covalent Binding and Fluorimetric Determination of Dialdehydes Using Aminated Silica Nanoparticles and Ethylenediamine Fluoresceinстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 6 декабря 2018 г.
- Авторы: Filenko I.A., Golodukhina S.V., Usol’tseva L.O., Adamova E.M., Beklemishev M.K.
- Журнал: Journal of Analytical Chemistry
- Том: 72
- Номер: 9
- Год издания: 2017
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 977
- Последняя страница: 985
- DOI: 10.1134/S1061934817090040
- Аннотация: The possibility of formation of a ternary (sandwich) compound of a dialdehyde (malondialdehyde, glutaraldehyde, or glyoxal) with ethylenediamine f luorescein thiocarbamyl (EDF) and silica nanoparticles noncovalently modified with polyethyleneimine (SiO2/PEI) with the subsequent f luorimetric determination of the dialdehyde was demonstrated. The mixed Schiff base SiO2 /PEI–dialdehyde–EDF (sandwich) is formed in an acetic acid solution on heating ina water bath. The sandwich and the excess of SiO2/PEI were separated from the unreacted f luorophore by centrifugation; the precipitate was washed and resuspended in water, and the f luorescence of solution was measured (λex = 470 nm, λem = 520 nm). The duration of an ana-lytical cycle was no longer than a half-hour. The limit of detection of dialdehydes in pure water is 1 × 10–5 M, and the analytical concentration range is 2 × 10–5 – 3 × 10–4 M (for malondialdehyde). The repeatability RSDs in this concentration range were 3–5% (n= 3). The comparable concentrations of sulfamethoxazole, sulfadiazine, pyracetam, and chloramphenicol and 1 × 10–5 M of ceftriaxone, ceftazidime, metamizole (analgin), isoniazid, and amikacin caused no interference with the determination of 3 × 10–4 M malondial-dehyde; protein noticeably interfered. The determination of glyoxal and glutaraldehyde in disinfectants was carried out.
- Добавил в систему: Беклемишев Михаил Константинович