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Improved electrochemical analysis of neuropathy target esterase activity by a tyrosinase carbon paste electrode modified by 1-methoxyphenazine methosulfateстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Sokolovskaya L.G., Sigolaeva L.V., Eremenko A.V., Gachok I.V., Makhaeva G.F., Strakhova N.N., Malygin V.V., Richardson R.J., Kurochkin I.N.
- Журнал: Biotechnology Letters
- Том: 27
- Номер: 16
- Год издания: 2005
- Издательство: Springer Nature
- Местоположение издательства: Switzerland
- Первая страница: 1211
- Последняя страница: 1218
- DOI: 10.1007/s10529-005-0020-1
- Аннотация: A graphite-paste tyrosinase biosensor was improved by adding 1-methoxyphenazine methosulfate as a mediator. Mediator modification enhanced sensitivity to phenol 4-fold and long-term stability 3-fold. Phenol could be detected at 25 nM (S/N=2) using an Ag/AgCl reference electrode. The biosensor was used to measure the activity of a toxicologically significant enzyme, neuropathy target esterase (NTE), which yields phenol by hydrolysis of the substrate, phenyl valerate. Using the new biosensor, blood and brain NTE inhibition by organophosphorus (OP) compounds with different neuropathic potencies were well correlated (r=0.990, n=7), supporting the use of blood NTE as a biochemical marker of exposure to neuropathic OP compounds.
- Добавил в систему: Курочкин Илья Николаевич