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[A new method of isolation and purification of SsoII restriction endonuclease]статья
Информация о цитировании статьи получена из
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
- Авторы: Kariagina A.S., Levchenko I.I.a, Nikol’skaia I.I.
- Журнал: Biochemistry (Moscow)
- Том: 58
- Номер: 6
- Год издания: 1993
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 908
- Последняя страница: 912
- Аннотация: A new method for isolation and purification of restriction endonuclease SsoII which results in a homogeneous preparation suitable for all types of fine physico-chemical assays has been elaborated. The procedure includes four chromatographic steps: fractionation on butyl-Toyopearl, combined chromatography on SP-Toyopearl and phosphocellulose PII, and chromatography on DEAE-Toyopearl and on QAE-Toyopearl. The use of fast flow sorbents (Toyopearl) makes it possible to reduce the time needed for the separation of proteins and to optimize the fractionation conditions, thus avoiding the dialysis between the chromatographic steps which significantly decreased the enzyme activity yields in previous purification schemes. The isolation of restriction endonuclease SsoII by the new method usually takes four days.
- Добавил в систему: Карягина-Жулина Анна Станиславовна