The role of non-conservative Cys 62, 86, 146 and 164 residues in the functioning of Luciola mingrelica firefly luciferaseстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:Using the firefly luciferase L. mingrelica carrying C-terminal His6-tag (WT-His6) [4] the following mutant forms were obtained: C62S, C62V, C86S, C146S, C164S (single mutants), C62/146S, C62/164S, C86/146S, C146/164S (double mutants) and C62/146/164S (triple mutant) (Fig. 1). Comparison of the properties of mutant forms obtained on the basis of WT and WT-His6-luciferases revealed that the introduction of His6-tag affected the kinetics of thermal inactivation, eliminating the first (fast) step and thus leading to the one-step process of inactivation and the increase of thermal stability. No concentration dependence of stability was observed for any forms of His6-luciferase.