Self-diffusion of water-soluble fullerene derivatives in mouse erythrocytes

Avilova, I.; Khakina, E.; Kraevaya, O.; Kotelnikov, A.; Kotelnikova, R.; Troshin, P.; Volkov, V.

Статья опубликована в высокорейтинговом журнале

Информация о цитировании статьи получена из Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 29 мая 2019 г.

Авторы: Avilova Irina, Khakina Ekaterina, Kraevaya Ol'ga, Kotelnikov Alexander, Kotelnikova Raisa, Troshin Pavel, Volkov Vitaliy
Журнал: Biochimica et Biophysica Acta - Biomembranes
Том: 1860
Год издания: 2018
Издательство: Elsevier BV
Местоположение издательства: Netherlands
Первая страница: 1537
Последняя страница: 1543
DOI: 10.1016/j.bbamem.2018.05.007
Аннотация: Self-diffusion of water-soluble fullerene derivative (WSFD) C60[S(CH2)3SO3Na]5H in mouse red blood cells (RBC) was characterized by 1H pulsed field gradient NMR technique. It was found that a fraction of fullerene molecules (~13% of the fullerene derivative added in aqueous RBC suspension) shows a self-diffusion coefficient of (5.5 ± 0.8)·10−12m2/s, which is matching the coefficient of the lateral diffusion of lipids in the erythrocyte membrane (DL= (5.4 ± 0.8)·10−12 m2/s). This experimental finding evidences the absorption of the fullerene derivative by RBC. Fullerene derivative molecules are also absorbed by RBC ghosts and phosphatidylcholine liposomes as manifested in self-diffusion coefficients of (7.9 ± 1.2)·10−12 m2/s and (7.7 ± 1.2)·10−12 m2/s, which are also close to the lateral diffusion coefficients of (6.5 ± 1.0)·10−12m2/s and (8.5 ± 1.3)·10−12 m2/s, respectively. The obtained results suggest that fullerene derivative molecules are, probably, fixed on the RBC surface. The average residence time of the fullerene derivative molecule on RBC was estimated as 440 ± 70 ms. Thus, the pulsed field gradient NMR was shown to be a versatile technique for investigation of the interactions of the fullerene derivatives with blood cells providing essential information, which can be projected on their behavior in-vivo after intravenous administration while screening as potential drug candidates.
Добавил в систему: Котельников Александр Иванович

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Self-diffusion of water-soluble fullerene derivatives in mouse erythrocytesстатья

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