Catalytic sites of hydrolases: Structure and catalytic cycleстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
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Scopus
Статья опубликована в журнале из перечня ВАК
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:The review is devoted to the analysis of published data on the structure of active sites and mechanisms of action of hydrolases. The bioinformatics methods employed for automated analysis of proteins are described, including contemporary protein databases and methods for classification and search for enzyme active sites. It is shown that the catalytic sites of the known hydrolases belong to one of the four principal types as regards the mechanism of water activation. By examples of glycosyl hydrolases and serine hydrolases it is demonstrated how different proteins employ common catalytic centres and mechanisms of action. The modes employed by catalytic centers for water activation are discussed and representative schemes of reactions are given for each type of catalytic sites. The role of aspartic acid and histidine in the substrate activation and the role of glycine, proline and cysteine in structural organization of catalytic sites are discussed. The rate and nature of limiting steps of catalytic cycles are discussed.