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Pannexin 1 in vasculature: interaction with purinergic signal systemстатья Тезисы
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 26 июня 2019 г.
- Авторы: Tarasova OS, Kiryukhina OO, Gaynullina DK, Panchin YuV, Shestopalov VI
- Журнал: Neurochemical Journal
- Том: 12
- Номер: 4 (Supplement)
- Год издания: 2018
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: S90
- Последняя страница: S90
- DOI: 10.1134/S1819712418040050
- Аннотация: Pannexins were discovered in the genome of vertebrates in 2000 as homologs of innexins, invertebrate gap junction proteins (Panchin et al. Curr Biol 2000, 10: R473-4). In vertebrates, pannexin channels represent a key pathway for ATP release from the cells and pannexin 1 is the most common (Panx1). Our work was aimed to study the vasomotor responses in mice with global Panx1 knockout as compared to wild type mice. Experiments were performed on saphenous and basilar arteries using wire myography and quantitative PCR techniques. For the first time, we explored Panx1 role in functioning of vascular endothelium and showed that the impact of Panx1 may depend on arterial type and diameter, Panx1 content and localization in the vascular wall as well as on the development of compensatory alterations in purinergic system. Panx1 content in cerebral arteries is much higher compared to peripheral arteries. In Panx1-knockout mice, relaxatory influence of the endothelium is decreased in relatively large saphenous artery but increased in relatively small basilar artery. In basilar, but not saphenous artery knockout of Panx1 gene is associated with compensatory augmentation of contractile and relaxatory responses to ATP, increased content of P2Y1-receptors mRNA and decreased content of CD39 ectonucleotidase mRNA. In basilar artery of wild type mice, ATP hydrolysis with the use of apyrase decreases endothelium-dependent relaxation under normal conditions, but increases it under conditions of hypercapnic acidosis, which is presumably due to altered contribution of P2Y-purinoceptor subtypes in ATP vasomotor effects. Such effects of apyrase are absent in Panx1-knockout mice. This suggests the involvement of Panx1 in cerebral vasoregulation both in normal conditions and during acidification of brain tissue. Supported by the Russian Science Foundation (project N17-15-01433).
- Добавил в систему: Тарасова Ольга Сергеевна