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One more probable structural transition in potato virus X virions and a revised model of the virus coat protein structureстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Nemykh M.A., Efimov A.V., Novikov V.K., Orlov V.N., Arutyunyan A.M., Drachev V.A., Lukashina E.V., Baratova L.A., Dobrov E.N.
- Журнал: Virology
- Том: 373
- Номер: 1
- Год издания: 2008
- Издательство: Academic Press
- Местоположение издательства: United States
- Первая страница: 61
- Последняя страница: 71
- DOI: 10.1016/j.virol.2007.11.024
- Аннотация: We found that a 2-h incubation of potato virus X (PVX) virions in 10 mM Tris-HCl buffer pH 7.5 at -20 degrees C results in a strong but reversible drop in virion stability. Under these conditions, the PVX virions are completely disrupted by low (starting from 50 mM) concentrations of LiCl and CaCl(2) but not of NaCl. Incubation of PVX samples with 0.05-2 M LiCl at +4 degrees C did not result in virion disassembly and the virions were not disrupted upon incubation at -20 degrees C in 10 mM Tris-HCl buffer pH 7.5 without LiCl. We suggest that a 2-h incubation of the PVX virions at -20 degrees C in 10 mM Tris-HCl pH 7.5 results in a structural transition in the virus particles. A revised model of the three-dimensional organization of coat protein subunits in the PVX virions is proposed. This two-domain model explains better the high plasticity of the PVX CP structure.
- Добавил в систему: Орлов Виктор Николаевич