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Approaches to engineer stability of beetle luciferasesстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
- Авторы: Koksharov Mikhail I., Ugarova Natalia N.
- Журнал: Computational and Structural Biotechnology Journal
- Том: 2
- Номер: 3
- Год издания: 2012
- Первая страница: e201209004
- DOI: 10.5936/csbj.201209004
- Аннотация: Luciferase enzymes from fireflies and other beetles have many important applications in molecular biology, biotechnology, analytical chemistry and several other areas. Many novel beetle luciferases with promising properties have been reported in the recent years. However, actual and potential applications of wild-type beetle luciferases are often limited by insufficient stability or decrease in activity of the enzyme at the conditions of a particular assay. Various examples of genetic engineering of the enhanced beetle luciferases have been reported that successfully solve or alleviate many of these limitations. This mini-review summarizes the recent advances in development of mutant luciferases with improved stability and activity characteristics. It discusses the common limitations of wild-type luciferases in different applications and presents the efficient approaches that can be used to address these problems.
- Добавил в систему: Кокшаров Михаил Иванович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Иллюстрация | Fig. 2. Typical non-lethal in vivo screening of the 90 mm plate (A) with mutant E. coli colonies for thermostability. In vivo bioluminescence before (B) and after (C) incubation of the plate at 50°C. The thermostable mutant is marked by the arrow. | Fig2.tif | 4,0 МБ | 11 ноября 2012 [koksharov83] |
| 2. | Иллюстрация | Fig. 1. 3D structure of beetle luciferases | Fig1.tif | 399,5 КБ | 11 ноября 2012 [koksharov83] |
| 3. | Полный текст | Koksharov_Ugarova_stability-mini-review_2012.pdf | 645,3 КБ | 9 августа 2015 [koksharov83] |